Purification of trypsin inhibitor from seeds of Cicer arietinum (L.) and its insecticidal potential against Helicoverpa armigera (Hübner)

نویسندگان

  • Meera Nair
  • Sardul Singh Sandhu
  • Anita Babbar
چکیده

INTRODUCTION Cicer arietinum is an important self-pollinated diploid crop 2n=2x=16 with genome sized approximately 931 Mbp (Arumuganathan and Earle 1991). India is the largest producer of chickpea, contributing with about 66% of the total global production, followed by Turkey, Pakistan and Mexico (Thangwana and Ogola 2012). It is a major source of protein (12.4-31.5%), energy, fiber, vitamins, minerals and essential amino acids such as tryptophan and lysine (Maiti 2001). Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) is a polyphagous pest of 182 plant species across 47 families in the Indian subcontinent, out of which 56 are heavily damaged and 126 are rarely affected. Helicoverpa armigera (Hübner), also known as cotton bollworm or legume pod borer, is one of the most devastating crop pest of Cicer arietinum since it inflicts annual losses of over US$ 328 million. Losses caused only by this pest reported up to US$ 17 million in crops like cotton, pigeon pea, chickpea, groundnut, sorghum, pearl millet, tomato and others ABSTRACT : Trypsin inhibitors (TI) have crucial functions in plant defense against pests, and recent studies reveal their diversity in stress response. Disease and herbivore infestations are harmful to plant growth, but the response of TI in Cicer arietinum (L.) in Central India has been less studied. In the present study, we partially characterized the TI from Cicer arietinum (L.) seeds and evaluated its insecticidal potential against Helicoverpa armigera (Hübner). A 20 kDa trypsin inhibitor was purified to homogeneity by ammonium sulfate precipitation and chromatographies with Sephadex G-100 and diethylaminoethyl cellulose (DEAE-cellulose-52) ion-exchange column. TI assay categorized 17 cultivars in three groups: with high (more than 70%), intermediate (16–70%) and very low (0–15%) TI activity. Moreover, results from the TI activity coincided with herbivore attack studies carried out in J.N.K.V.V. fields earlier. Together, our results suggest that the presence of TI triggers a signal that leads the dynamics of stress response and, in turn, regulates pest resistance in plants. Feeding experiments conducted with 5th instar larvae suggested dose-dependent decrease for both the larval weight and the survival of the larva.

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Purification and characterization of trypsin inhibitor from Cicer arietinum L. and its efficacy against Helicoverpa armigera

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تاریخ انتشار 2013